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Table 1 Species collected at field site as determined by individual identification and 454 pyrosequencing

From: Environmental monitoring using next generation sequencing: rapid identification of macroinvertebrate bioindicator species

Species Field sites
  BR08 DB09 GC09 HW09 LE09 MC09 ME09 RL09 SK09 UK09
  n (reads) n (reads) n (reads) n (reads) n (reads) n (reads) n (reads) n (reads) n (reads) n (reads)
Chironominae
Chironomus australis        100 (5318) 1 (318) 0 (2) 18 (2370) 1 (394) 1 (8) 71 (5519)
Chironomus cloacalis        18 (998) 11 (1580)    1 (79) 1 (23)    5 (511)
Chironomus duplex        6 (405) 3 (149)    1 (253) 3 (225)    6 (2327)
Chironomus februarius        22 (1104) 6 (821)      20 (861)   (1) 2 (82)
Chironomus nepeanensis                    1 (298)
Chironomus oppositus 3 (35) 2 (603) 1 (934)    57 (6197) 2 (815)    22 (780) 32 (4803) 1 (0)
Chironomus pseudoppositus                3 (96)     
Chironomus tepperi          3 (166)      1 (6)     
Cladopelma sp.1          0 (4) 2 (869)        1 (10)
Cladopelma sp.2                8 (292)     
Cladotanytarsus australomancus    1 (16)        14 (4239)         
Cladotanytarsus sp.C 1 (7)                   
Dicrotendipes pseudoconjunctus    4 (999)    3 (100) 6 (479) 1 (1064) 21 (4724) 6 (198) 1 (579)   
Dicrotendipes septemmaculatus    0 (1)    1 (17)             
Dicrotendipes sp.4                1 (18) 2 (895)   
Dicrotendipes sp.A      2 (1877)               
Kiefferulus cornishi      1 (686)        1 (29)    4 (516) 4 (75)
Kiefferulus intertinctus    7 (581) 3 (1171) 1 (30) 2 (32)    1 (177)    4 (1737) 1 (236)
Kiefferulus martini          1 (168)      1 (18)     
Microchironomus forcipatus                1 (7)     
Parachironomus delinificus 1 (430)        1 (10)           
Parachironomus sp.3 3 (324)                   
Paratanytarsus grimmii      1 (308)    3 (264)        5 (1483)   
Paratanytarsus sp.D              1 (7)       
Polypedilum convexum 3 (332)                   
Polypedilum nubifer        9 (401)            1 (63)
Polypedilum sp.C    43 (2367)    0 (2)    2 (929)         
Polypedilum sp.E            1 (284)        1 (9)
Riethia stictoptera 1 (913) 6 (905)                 
Tanytarsus inextentus    1 (22)            1 (5)     
Tanypodinae
Ablabesmyia sp.2 1 (46)                   
Coelopynia sp.1 1 (26)        0 (1)           
Paramerina sp.4    1 (74)                 
Procladius paludicola 11 (1702) 9 (349)    1 (18)    0 (1)        2 (95)
Procladius sp.1    4 (1382)                 
Procladius sp.2 39 (4609)    0 (4)               
Procladius villosimanus        5 (664) 2 (285)    2 (1392) 30 (6660)     
Orthocladiinae
Botryocladius sp.1 1 (96)                   
Corynoneura scutellata              2 (16)    1 (3)   
Cricotopus albitarsis      4 (156)               
Cricotopus annuliventris              1 (27)       
Cricotopus sp.1      1 (3)               
Cricotopus sp.2 4 (31)    19 (3930)      6 (1009)         
Paralimnophyes sp.1          2 (69)    2 (27)      0 (2)
Paratrichocladius sp.1        1 (6)             
Paratrichocladius sp.2                  1 (211)   
Total number of individuals 69   78   32   167   98   28   51   99   50   96  
Total number of reads   8551   7299   9069   9063   10543   9212   9101   9583   10236   9227
Total number of species 12   10   8   11   13   7   11   14   9   12  
  1. The number of individuals collected in this study at each field site (n) and the number of sequences (reads) from the 454 pyrosequencing experiments representing each species in parentheses. Differences in species detected by individual identification and by 454 pyrosequencing are bolded.