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Table 1 Species collected at field site as determined by individual identification and 454 pyrosequencing

From: Environmental monitoring using next generation sequencing: rapid identification of macroinvertebrate bioindicator species

Species

Field sites

 

BR08

DB09

GC09

HW09

LE09

MC09

ME09

RL09

SK09

UK09

 

n

(reads)

n

(reads)

n

(reads)

n

(reads)

n

(reads)

n

(reads)

n

(reads)

n

(reads)

n

(reads)

n

(reads)

Chironominae

Chironomus australis

      

100

(5318)

1

(318)

0

(2)

18

(2370)

1

(394)

1

(8)

71

(5519)

Chironomus cloacalis

      

18

(998)

11

(1580)

  

1

(79)

1

(23)

  

5

(511)

Chironomus duplex

      

6

(405)

3

(149)

  

1

(253)

3

(225)

  

6

(2327)

Chironomus februarius

      

22

(1104)

6

(821)

    

20

(861)

 

(1)

2

(82)

Chironomus nepeanensis

                  

1

(298)

Chironomus oppositus

3

(35)

2

(603)

1

(934)

  

57

(6197)

2

(815)

  

22

(780)

32

(4803)

1

(0)

Chironomus pseudoppositus

              

3

(96)

    

Chironomus tepperi

        

3

(166)

    

1

(6)

    

Cladopelma sp.1

        

0

(4)

2

(869)

      

1

(10)

Cladopelma sp.2

              

8

(292)

    

Cladotanytarsus australomancus

  

1

(16)

      

14

(4239)

        

Cladotanytarsus sp.C

1

(7)

                  

Dicrotendipes pseudoconjunctus

  

4

(999)

  

3

(100)

6

(479)

1

(1064)

21

(4724)

6

(198)

1

(579)

  

Dicrotendipes septemmaculatus

  

0

(1)

  

1

(17)

            

Dicrotendipes sp.4

              

1

(18)

2

(895)

  

Dicrotendipes sp.A

    

2

(1877)

              

Kiefferulus cornishi

    

1

(686)

      

1

(29)

  

4

(516)

4

(75)

Kiefferulus intertinctus

  

7

(581)

3

(1171)

1

(30)

2

(32)

  

1

(177)

  

4

(1737)

1

(236)

Kiefferulus martini

        

1

(168)

    

1

(18)

    

Microchironomus forcipatus

              

1

(7)

    

Parachironomus delinificus

1

(430)

      

1

(10)

          

Parachironomus sp.3

3

(324)

                  

Paratanytarsus grimmii

    

1

(308)

  

3

(264)

      

5

(1483)

  

Paratanytarsus sp.D

            

1

(7)

      

Polypedilum convexum

3

(332)

                  

Polypedilum nubifer

      

9

(401)

          

1

(63)

Polypedilum sp.C

  

43

(2367)

  

0

(2)

  

2

(929)

        

Polypedilum sp.E

          

1

(284)

      

1

(9)

Riethia stictoptera

1

(913)

6

(905)

                

Tanytarsus inextentus

  

1

(22)

          

1

(5)

    

Tanypodinae

Ablabesmyia sp.2

1

(46)

                  

Coelopynia sp.1

1

(26)

      

0

(1)

          

Paramerina sp.4

  

1

(74)

                

Procladius paludicola

11

(1702)

9

(349)

  

1

(18)

  

0

(1)

      

2

(95)

Procladius sp.1

  

4

(1382)

                

Procladius sp.2

39

(4609)

  

0

(4)

              

Procladius villosimanus

      

5

(664)

2

(285)

  

2

(1392)

30

(6660)

    

Orthocladiinae

Botryocladius sp.1

1

(96)

                  

Corynoneura scutellata

            

2

(16)

  

1

(3)

  

Cricotopus albitarsis

    

4

(156)

              

Cricotopus annuliventris

            

1

(27)

      

Cricotopus sp.1

    

1

(3)

              

Cricotopus sp.2

4

(31)

  

19

(3930)

    

6

(1009)

        

Paralimnophyes sp.1

        

2

(69)

  

2

(27)

    

0

(2)

Paratrichocladius sp.1

      

1

(6)

            

Paratrichocladius sp.2

                

1

(211)

  

Total number of individuals

69

 

78

 

32

 

167

 

98

 

28

 

51

 

99

 

50

 

96

 

Total number of reads

 

8551

 

7299

 

9069

 

9063

 

10543

 

9212

 

9101

 

9583

 

10236

 

9227

Total number of species

12

 

10

 

8

 

11

 

13

 

7

 

11

 

14

 

9

 

12

 
  1. The number of individuals collected in this study at each field site (n) and the number of sequences (reads) from the 454 pyrosequencing experiments representing each species in parentheses. Differences in species detected by individual identification and by 454 pyrosequencing are bolded.
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Frontiers in Zoology

ISSN: 1742-9994