TEM section 3D registration. A. Template from an LM section that was used for TEM re-sectioning. B. TEM image co-registered to the LM template in Photoshop. TEM image layer set to 50% opacity. C. Registered TEM image with (nearly) black background as re-imported into AMIRA®. D. Voxel dimensions (0.413×0.413 μm in X and Y axes) of the cropped LM template before resampling to TEM resolution. E. Voxel dimensions (0.025×0.025 μm) of the cropped LM template after resampling to TEM resolution (same as in 3D registered TEM image).