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Table 1 Primers used for PCR and quantitative PCR amplification of ‘ Ca . S. philanthi’ genes

From: Dynamics of symbiont-mediated antibiotic production reveal efficient long-term protection for beewolf offspring

Primer

Sequence (5′-3′)

Direction

Ampl icon length

Reference

Strep_phil_fwd3mod

TGGTTGGTGGTGGAAAGC

Fwd

135

Kaltenpoth et al., 2010

Strep_16S_rt_rev

GTGTCTCAGTCCCAGTGTG

Rev

 

Kaltenpoth et al., 2010

PKS1-SPT-F1

TCTTCCGACAGTCGATAGCC

Fwd

139

This study

PKS1_SPT-R1

GAGATCATGACGGCGAAGAG

Rev

 

This study

Strep_phil_EF-Tu_rt_fwd

CGACTACACGCACAAGAAG

Fwd

108

This study

Strep_phil_EF-Tu_rt_rev

CACGGACGGGATGTACTC

Rev

 

This study.

Strep-phil-gyrB-rt_fwd

CGCCAACACGATCCACAC

Fwd

115

Kaltenpoth et al., 2010

Strep-phil-gyrB-rt_rev

GTCCTTCTCCCGCAGCAG

Rev

 

Kaltenpoth et al., 2010

FtsZ-Ptr_F1

GACCGACTGCTGTCCATTTC

Fwd

136

This study

FtsZ-Ptr_R1

CGAAGTCCAGGTTGATCAGG

Rev

 

This study

whiB(189)-F1

CGAGCTGTTCTTCCCCATC

Fwd

104

This study

whiB(189)-R1

ACTGCAGGCACTCCTCCAT

Rev

 

This study