Table 1 Primers used in this study for Amdop2 double-stranded RNA synthesis, reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analyses and construction of luciferase reporter vector
From: MicroRNA ame-let-7 targets Amdop2 to increase sucrose sensitivity in honey bees (Apis mellifera)
Gene | Application | Primer sequence (5′–3′) | Amplicon size (bp) | TM (°C) |
|---|---|---|---|---|
Amdop2 | RNA interference | F: CAGGCCTGGCTATACTCCTG R: GTCGGTGATGGCCCAGTA | 314 | 55 |
T7F: CAGGCCTGGCTATACTCCTG T7R: GTCGGTGATGGCCCAGTA | 350 | 58 | ||
Amdop2 | qPCR | F: CAAGACGTTGGGGATCGTGA R: GATCCAACCCAGCCACGTAA | 142 | 55 |
β-actin | F: TGCCAACACTGTCCTTTCTG R: AGAATTGACCCACCAATCCA | 138 | 55 | |
GAPDH | F:CACCTTCTGCAAAATTATGGCG R:ACCTTTGCCAAGTCTAACTGTTAA | 156 | 55 | |
ame-let-7 | F: GCATGTGAGGTAGTAGGTTG R: GTGCAGGGTCCGAGGT | 21 | 55 | |
Amdop2-CDS | Luciferase reporter assay | F: GAGTAAGGCGGCGGTATCAA R: TTTGCTCGCACGAACTCTCT | 420 | 59 |