Fig. 3

Internal morphologies in stadium V juveniles at the late preparatory and rigidation periods. A A histological section of the tail end in the sagittal plane of an individual in the late preparatory period stained with hematoxylin and eosin. B Higher magnification of the black boxed region in A. C The tail end of an individual in the rigidation period fixed in Bouin’s solution. D A histological section in transverse planes of a transparent protrusion in the rigidation period. Arrowheads indicate newly added legs elongating in the transparent protrusion. Note that in C, only two legs per transparent protrusion are visible because the left and right legs overlap. Scale bars show 200 μm (A–C), 50 μm (D). am, arthrodial membrane; l, legs; lp, leg primordia; ps, preanal sclerite; r, ring (each ring number is added after “r”)