Table 1 Names and sequences of primers used in the experiment
From: Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis
Primer name | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
DIB | CGCGGATCCGAAGCGACTAATGCAATCGC | CCGCTCGAGTTCGGGACCGTTTGTTGGA |
EGFP | CGCGGATCCATGGTGAGCAAGGGCGAGG | CCGCTCGAGTTACTTGTACAGCTCGTCCATGCCG |
qDIB | ATACTTCGGACGGATAATG | CAACGCAATACTCTCAATG |
qEGFP | CGCACCATCTTCTTCAAG | GTGGCTGTTGTAGTTGTAC |
EcR | GAGGCGCTGCAGGCTTAC | GAGTTTGGCAAACTCCGTCATC |
USP | GTTGGAGTCAAGGATGGTATCGT | AGCCGAGTTCCGGTGGAT |
E75 | TCCGGAGAAGTATTCAACAAAAGA | TGCGAAGAATGGAGCACTGT |
HR3 | AGTCATCACCTGCGAGGGC | GAACTTTGCGACCGCCG |
FTZ-f1 | ATCGTGCAAGGGATTCTTCA | ATCAGCGACGCAAGAATAGG |
GAPDH | TCGTCTCCAATGCTTCTT | CGGTCCATCAACAGTCTT |