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Table 1 Summary statistics for the cell- and nuclei dataset

From: Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material

Dataset

scRNAseq

snRNAseq

Estimated number of cells (# of discs)

14,487 (28)

9048 (41)

Number of cells per disc (% of expected number of cellsa)

517 (1.1)

221 (0.5)

Median genes per cell

537

812

Mean reads per cell

13,303

13,334

Valid barcodes

96.10%

97.10%

Number of reads

192,731,871

120,649,741

Fraction of reads in cells

38.20%

73.50%

Total genes detected

11,062

12,296

Median UMI counts per cell

1249

1383

Reads mapped to genome

93.20%

85.70%

Reads mapped confidently to genome

86.40%

84.50%

Reads mapped confidently to intronic regions

2.10%

14.70%

Reads mapped confidently to intergenic regions

9.00%

0.80%

Reads mapped confidently to exonic regions

75.20%

69.00%

Percentage of cells with high mitochondrial read count (> 10%)

14.00%

0.02%

  1. aAssuming that a late third instar eye-antennal disc is composed of about 44,000 cells[49]