Fig. 2
From: MiR-34b/c play a role in early sex differentiation of Amur sturgeon, Acipenser schrenckii
miR-34b/c expression in gonads during sex differentiation of A. schrenckii. A, B Spatiotemporal expression patterns of miR-34b and miR-34c using real-time PCR. The relative expression levels of miR-34b/c were normalized against the UGs (undifferentiated gonads, i.e. 5 M) as reference samples. *Represents P < 0.05 with a statistically significant difference between the two groups. Subsequently, location expression of miR-34c were detected in ovaries (C–F) and testes (G, H) of 36 M Amur sturgeon individuals using in situ hybridization (miISH). C, G were negative controls detected for hybridization with blank miRNA hybridization buffer. OI, ovarian lamellae; PG, primary growth oocyte; nu, oocyte nucleoli; GRC, granulosa cell; OG, oogonia; SG, spermatogonia; SC, Sertoli cell; LC, leydig cell.. Scale bar in C and D = 100 μm and in E, F, G and H = 50 μm