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Fig. 4 | Frontiers in Zoology

Fig. 4

From: Generation and characterization of genome-modified chondrocyte-like cells from the zebra finch cell line immortalized by c-MYC expression

Fig. 4

Targeted knockout of the SOX9 gene in zebra finch chondrocyte-like cells (CLCs). A Schematic illustration showing the positions of the gRNAs used to target the zebra finch SOX9 gene. B Sequences of the gRNAs and structure of the CRISPR/Cas9 plasmid targeting the SOX9 gene. C DNA sequences of the wild type and mutated SOX9 loci in transfected cells. Blue letters indicate gRNA recognition sequences, purple letters indicate PAM sequences, and gray letters indicate deletions. D The expression levels of chondrocyte marker genes in control (wild type) and SOX9 knockout CLCs; **p < 0.01, ***p < 0.001. E, F Detection of apoptotic control and SOX9 knockout CLCs. The cells were treated with the TUNEL reaction mixture (TMR red labeling) and apoptotic cells were counted manually. Scale bar, 50 μm; ****p < 0.0001

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