Table 2 Antibodies/antisera, research resource identifiers and dilutions

Primary

 Mouse anti-SYNORF1 (Drosophila synapsin-1), monoclonal, supernatant, Developmental Studies Hybridoma Bank, #3C11

AB_528479

1:100

  Mouse anti-acetylated tubulin IgG 2b isotype, clone 6-11 B-1, Sigma-Aldrich, #T6793

AB_477585

1:200–300

 Rat anti-alpha tubulin IgG2a isotype, clone YL1/2, Thermo Fisher Scientific, #MA1-80017

AB_2210201

1:500

Secondary

 Cy3-AffiniPure goat anti-mouse IgG (H + L), polyclonal, Jackson ImmunoResearch Labs, #115-165-166

AB_2338692

1:250

 Alexa Fluor647-AffiniPure goat anti-rat IgG (H + L), polyclonal Jackson ImmunoResearch Labs, #112-605-167

AB_2338404

1:250