Fig. 9

Stage 11 (A–B′, E–F′) and stage 12 (C–D′, G–G′). In situ hybridization for Popi-en (A–D′) and confocal micrographs of fluorescent antibody staining against tropomyosin (F′, G′). A–B′: Fifth opisthosomal segment and initial folding of the anterior furrow. C–D′: Sixth opisthosomal segment. E: Detail of the head lobes of early stage 11. F: Detail of the head lobes of late stage 11 embryo. F′: Maximum projection of a flat-mounted late stage 11 embryo (ventral view). G: Detail of the head lobes of a stage 12 embryo. G′: Maximum projection of a flat-mounted embryo at stage 12 (ventral view). A and P: anterior–posterior axis; arrowhead: stripe of engrailed expression marking the posterior border of opisthosomal segment; blue circle: Hoechst (blue, gray); white circle: bright field; yellow circle: tropomyosin (yellow); asterisk: anterior rim of the folding ectoderm; af: anterior furrow; ch: cheliceral segment; lr: labrum; lf: lateral furrow; L1–4: leg 1–4 segments; mb: medial bridge; pp: pedipalpal segment; s: slit; yk: yolk. Scale bars A–G: 100 µm. F′, G′: 250 µm