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Fig. 7 | Frontiers in Zoology

Fig. 7

From: Eggs to long-legs: embryonic staging of the harvestman Phalangium opilio (Opiliones), an emerging model arachnid

Fig. 7

Stage 9 (A–D′). In situ hybridization for the gene Popi-engrailed (A–B′) and confocal micrographs of fluorescent antibody staining against tropomyosin (C) and alpha-tubulin (D, D′). Images with the same letters are different views of the same embryo. A–B′: Third opisthosomal segment. C: Maximum projection of a flat-mounted embryo (ventral view). Tropomyosin-positive cells occur as a bundle inside each prosomal limb buds and in broad stripes in each opisthosomal segment and posterior growth zone. D: Maximum projection of a flat-mounted embryo (ventral view). Note groups of invaginating cells along ventral ectoderm and head lobes. D′: Detail of a group of invaginating neural precursor cells on the ventral ectoderm. A and P: anterior–posterior axis; arrowhead: stripe of engrailed expression marking the posterior border of opisthosomal segment; black arrow: neural precursor cells (apical process); blue circle: Hoechst (blue); white arrow: isolated tropomyosin-positive cell; white circle: bright field; yellow circle: tropomyosin (yellow); green circle: alpha-tubulin (green); ch: cheliceral segment; gc: germ cell cluster; L1–4: leg 1–4 segments; pp: pedipalpal segment; st: stomodeum. Scale bars A–B′: 100 µm. C, E: 250 µm. D reprinted with permission from Sharma (2018) Current Biology 28, R774–R778

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