Fig. 5

Germ cell development in P. opilio. A, D: In situ hybridization for Popi-vasa. B–C: Double in situ hybridization for Popi-vasa and Popi-en. E–E′: Antibody staining for tropomyosin. A: Stage 6 embryo. B: Stage 9 embryo. C: Stage 11 embryo. D: Stage 14 embryo. E: Single optical slice through the germ cell cluster at stage 9, with orthogonal projections. E′: Detail of the germ cell nuclei shown in “E”. A and P: antero-posterior axis; blue circle: Hoechst; orange circle: tropomyosin; white circle: bright field; ech: ectodermal cell chromatin; gc: germ cell cluster; gch: germ cell chromatin; gz: growth zone; O3–O7: opisthosomal segments 3–7. Scale bar A–D: 100 µm; E: 50 µm; E′: 25 µm