Fig. 4

Stage 5 (A) and early (B–D′) to late (E–E′) stage 6. In situ hybridization for Popi-en. A, A′: Early germ band. No clear expression of Popi-en was detected. B–D′: Initial segmentation of the prosoma. D–E′: C-shaped segmented germ band longer in the A–P axis and with more defined head-lobe. A and P: anterior–posterior axis; asterisk: dot of engrailed expression; D and V: dorsal–ventral axis; white arrow: horseshoe shaped posterior end of the germ band; blue circle: Hoechst staining. white circle: bright field; ch: cheliceral segment; L1–4: leg 1–4 segments; oc: ocular segment; pp: pedipalpal segment. Scale bars: 100 µm