Table 1 Plectrohyla species used for the different methods applied
P. sagorum | P. matudai | P. hartwegi | |
|---|---|---|---|
Behavioural observations (mating) | ✓ | ||
Teeth length comparison between males and females | ✓ | ✓ | ✓ |
Distance between male teeth vs. Female scratches | ✓ | ✓ | |
Microscopic structures of lip glands | ✓ | ✓ | ✓ |
Lip gland protein expression and phylogenetic analysis | ✓ | ✓ |