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Fig. 9 | Frontiers in Zoology

Fig. 9

From: Revisiting kinorhynch segmentation: variation of segmental patterns in the nervous system of three aberrant species

Fig. 9

Serotonin-LIR in the nervous system of Zelinkaderes yong. Anterior is up in all panels. A Schematic representation, ventral view. Grey shading on the left side marks the different body regions. B–F Confocal Z-stack projections of specimens co-labeled with 5HT (B–F), and DAPI (F). Autofluorescence of the cuticle was kept as guidance. Colour legend in (B) applies to (C–E). B 5HT-LIR overview, ventral view. C Segments 1-5, lateral view, left side, introvert withdrawn. D Detail of brain neuropil and associated somata, dorsal view. E Segments 8-9, detail of the ventral nerve cord 5HT-LIR neurites and lateroventral 5HT-LIR somata, ventral view. F Segments 3-5, detail of ventral nerve cord and associated 5HT-LIR somata, ventral view. Arrows mark the position of the immunoreactive somata of segment 9 in (B, E). Dashed circles mark the position of the 5HT-LIR somata associated to the brain in (B–D). Scale bars: 20 μm. Abbreviations: asb, anterior somata of the brain; cne, convergent neurite; cnr, 5HT-LIR complete nerve ring; i, introvert; inr, 5HT-LIR incomplete nerve ring; mcnr, mouth cone nerve ring; n, neck; np, neuropil; t, trunk; tn, transverse neurite; vln, ventrolateral nerve; vms, ventromedial somata of the brain; vnc, ventral nerve cord; vncs, ventral nerve cord somata; vncn, ventral nerve cord neurite. Numbers refer to segments

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