Fig. 8

Acetylated α-tub-LIR in the nervous system of Zelinkaderes yong. Anterior is up in all panels. A, B Schematic representation in dorsal (A) and ventral (B) view. Grey shading on the left side marks the different body regions. For clarity, innervation of the introvert scalids have been omitted. Only the base of each of the middorsal spines is represented in (A). C–J Confocal Z-stack projections of specimens co-labelled with acetylated α-tubulin (C–J) and DAPI (C–F, I, J). Autofluorescence of the cuticle was kept for guidance. Colour legend in (C) applies to all panels. C Segments 1-4 ventral view, introvert retracted. Dotted areas mark the position of the ventral nerve cord ganglia. Note the length of the ganglia between segment 2-3 and absence of ganglia in segment (1) D Detail of the brain, convergent neurites and ventral nerve cord, segments 1-3, introvert retracted. E Segments 7-9 showing the ventral nerve cord, ganglia and transverse neurites. Brackets mark the limits of each segment. Note the position of some ganglia and origin of transverse neurites is intersegmental. F Segments 3-6, dorsal view. G Detail of the innervation of the middorsal spines and sensory spots of segments 4-6, dorsal view. H.Same specimen as in (C) detail of segments 2-3, ventral view, note the position of the transverse neurite of segment (2) I Detail of the innervation of cuspidate and acicular spines of segments 8-9, left side, ventral view. J Detail of the mouth cone innervation. Scale bars: 20 μm. Abbreviations: b, brain; cne, convergent neurite; cu, cuspidate spine; cun, cuspidate spine neurite; i, introvert; isn, inner oral style neurite; lnb, longitudinal neurite bundle; mc, mouth cone; mcnr, mouth cone nerve ring; mds, middorsal spine; mts, midterminal spine; np, neuropil; osn, outer oral style neurite; sdn, subdorsal nerve; sn, spine neurite; ssn, sensory spot neurite; t, trunk; tn, transverse neurite bundle; tsn, lateral terminal spine neurite; vln, ventrolateral nerve; vnc, ventral nerve cord; vncn, ventral nerve cord neurite bundle. Numbers refer to segments