Fig. 3

Serotonin-LIR in the nervous system of Franciscideres kalenesos. Anterior is up in all panels. A Schematic representation, ventral view. Grey shading on the left side marks the different body regions. B–G Confocal Z-stack projections of specimens co-labelled with 5HT (B–G), acetylated α-tubulin (E–G) and DAPI (E, F). Autofluorescence of the cuticle was kept as guidance in (C, D). Colour legend in (C) applies to (D), colour legend in (E, F) also applies to (G). B Detail of brain neuropil, ventral nerve cord and associated somata, ventral view. C Detail of brain neuropil, ventral nerve cord and associated somata, lateral view. D Detail of brain neuropil and associated somata, dorsal view. E Introvert, neck and segments 1-3 ventral view. F same specimen as in (E), dorsal view. Arrows mark the position of the circular α-tub-LIR structures (2 ventral in E and 2 dorsal in F). G segments 8-11, ventrolateral view.Dashed circles mark the position of the 5HT-LIR somata associated to the brain in (B–F). Scale bars: 20 μm. Abbreviations: asb, anterior somata of the brain; cne, convergent neurite; cnr, 5HT-LIR complete nerve ring; i, introvert; inr, 5HT-LIR incomplete nerve ring; mcnr, mouth cone nerve ring; n, neck; np, neuropil; psb, posterior somata of the brain; spn, spinoscalid neurite; t, trunk; vms, ventromedial somata of the brain; vnc, ventral nerve cord; vncs, ventral nerve cord somata; vncn, ventral nerve cord neurite bundle. Numbers refer to segments