Fig. 2

Acetylated α-tub-LIR in the nervous system of Franciscideres kalenesos. Anterior is up in all panels. A, B. Schematic representation in dorsal (A) and ventral (B) view. Grey shading on the left side marks the different body regions. For clarity, innervation of the introvert scalids and the ring-like structures associated to the midventral part of the brain neuropil in (B) have been omitted. Only the base of each of middorsal spine is marked in (A). C–J. confocal Z-stack projections of specimens co-labelled with acetylated α-tubulin (C–J) and DAPI (E). Autofluorescence of the cuticle was kept as guidance in (C–J). Colour legend in (C) applies to panels (C, D, G–J). C Segments 1-5 ventrolateral view, introvert retracted. D Same specimen as in (C) in dorsal view. Arrowheads mark the position of the α-tub-LIR ring-like structures associated with the introvert. E, F Detail of segments 7-8 ventral view, dotted areas mark the position of the ganglia in the ventral nerve cord. G Segments 9-11 dorsal view. H Segments 10-11 ventral view. I Detail of segments 4-5, right side. Note the innervation of the lateroventral spines of segments 4-5. J Segments 6-7, dorsal view. Scale bars: 20 μm (C, D, J); 10 μm (E–I). Abbreviations: i, introvert; isn, inner oral styles neurite; lnb, longitudinal neurite bundle; mc, mouth cone; mcnr, mouth cone nerve ring; mds, middorsal spine; n, neck; np, neuropil; osn, outer oral style neurite; psn, primary spinoscalid neurite bundle; sdn, subdorsal nerve; sn, spine neurite; ssn, sensory spot neurite; t, trunk; tn, transverse neurite bundle; tsn, lateroterminal spine neurite; vln, ventrolateral nerve; vnc, ventral nerve cord; vncn, ventral nerve cord neurite bundle. Numbers refer to segments