Fig. 5
From: Improved histological fixation of gelatinous marine invertebrates
A range of gelatinous and soft-bodied animals fixed using ~ 16% Rain-X® in appropriate-strength FSW (full-strength FSW for all specimens except N. vectensis, which was fixed in 1/3X FSW), washed with PBST, and stored at 4 °C in PBST for 2 days – 2 weeks before imaging. a. Annelid worm, Capitella teleta adult. b. C. teleta juvenile. c. C. teleta larvae. d. Hydrozoan medusa. E. Hydractinia symbiolongicarpus primary polyp. f. Adult polyps of a colonial cnidarian. g. Nematostella vectensis primary polyp’s tentacles (DIC). h. The same sample as in (g) showing immunofluorescence pattern of acetylated tubulin (green) and nuclei (DAPI, blue). i. Hydrozoan medusa. j. Mysid. k. Copepod. l. Pelagic chaetognaths