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Fig. 2 | Frontiers in Zoology

Fig. 2

From: Improved histological fixation of gelatinous marine invertebrates

Fig. 2

EdU incorporation assay pattern is comparable to previous published observations with improved gross morphology. A-A”. Maximum intensity projection of an aboral view of a M. leidyi cydippid labeled with EdU in a pulse-chase experiment as in [3]. B-B″. Higher magnification view showing co-localization of EdU label and robust nuclear staining (DAPI) in the intact epidermis of the aboral surface. EdU signal (red, A’, B′) is concentrated in the paired tentacle bulbs and in isolated cells scattered throughout the epidermis. DAPI signal (blue, A”, B″) shows that the EdU signal co-localizes with a subset of nuclei

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