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Fig. 1 | Frontiers in Zoology

Fig. 1

From: Improved histological fixation of gelatinous marine invertebrates

Fig. 1

Morphological preservation of a range of M. leidyi samples. a. A live specimen of M. leidyi. b. The same animal as in A after fixation with only ~ 16% Rain-X® in filtered seawater (FSW) as detailed in protocol. Scale bar in (b) also applies to (a. c, d). A range of M. leidyi cydippid sizes (~ 0.5–8 mm) fixed according to the same protocol; every individual is adequately preserved across a range of body sizes. See Supplemental Fig. 1 A-C for a comparison with standard aldehyde fixation. e. A recently hatched M. leidyi cydippid (aboral view) fixed according to the modified protocol for small samples < 250 μm. f. A two-week old cydippid fixed according to the protocol and dehydrated into 100% methanol for long-term storage. g. Close-up of comb row and underlying gonad in a larger (2–3 mm) cydippid fixed according to the protocol. The aboral sense organ can also be seen in the lower right-hand corner of the image. h. Close-up view of tentacles of the same animal as in (g). The tentacles remain outside the body and some of the tentilla (fine projections of the tentacle) remain extended. Scale bar in H also applies to (g)

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