Fig. 2

Crystal structures and binding pockets of rat CBG (PDB code: 2v95) [18] and Zebra Finch CBG (PDB code: 5hgc) [83]. A, the native rat CBG-cortisol structure. B, the cortisol-binding pocket of rat CBG. C, the native Zebra Finch CBG-cortisol structure. D, the cortisol-binding pocket of Zebra Finch CBG. The β-sheets A, B, and C are in red, green, and blue, respectively. Helixes are in grey. Cortisol is shown as stick models and coloured in yellow. The cartoons are prepared using PYMOL (http://pymol.sourceforge.net). In rat CBG, residues that directly interact with cortisol are R10, A13, V17 (in hA); Q224, D226 (in s2B); T232, F234 (in s3B); R252, I255, D256, G259, K260 (in hH); F357, K359 (in s4B); W362 (in s5B). In Zebra Finch CBG, cortisol-interacting residues include V32, A36 (in hA); R246 (in s2B); T254, F256 (in s3B); K274, I277, Q278, S281 (in hH); F380 (in s4B); N385 (in s5B)