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Fig. 6 | Frontiers in Zoology

Fig. 6

From: When SEM becomes a deceptive tool of analysis: the unexpected discovery of epidermal glands with stalked ducts on the ultimate legs of geophilomorph centipedes

Fig. 6

Anatomy of ultimate legs in male Haplophilus subterraneus from semi-thin (a and b) and paraffin (c) sections. a Longitudinal section of the telopodite of an ultimate leg (composite), dorsomedial side to the top. The leg’s epidermis displays a massively developed glandular tissue: at the bottom (lateral to ventrolateral), secretory cells of mostly epidermal glands with stalked ducts are visible in sagittal section plane; whereas towards the top (medial to dorsomedial), secretory cells are cross-cut. The tendon and associated musculature traverse podomeres. Tarsus 1 possesses intrinsic musculature that terminates in the distal joint region of tarsus 1. Other muscles in tarsus 1 contribute to the tendon musculature (compare also Fig. 7d, e). Frequently, coagulated hemolymph is heavily stained (especially in tarsus 2). b Detail view of the distal tip of tarsus 2. This section reveals that the externally visible notch/depression (compare Fig. 5a-c) continues approx. 10 μm and connects with the tendon. c Sagittal section of the distal podomeres of the ultimate leg. Likewise, sagittal and cross section profiles of secretory cells of epidermal glands are visible (compare a). In this preparation, the association of stalked ducts and epidermal glands is evident. The cytoplasm of the glands is stained pale pink, nuclei and musculature red, and the hemolymph blue. Abbrevtiations: fe femur, imu intrinsic musculature of tarsus 1, inv terminal invagination, gl glandular tissue, he hemolymph, m medial, mu musculature, ta1 tarsus 1, ta2 tarsus 2, ten tendon, ti tibia, tfl tendon flexor, tmu tendon musculature

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