Fig. 5
From: Mitochondrial alternative oxidase contributes to successful tardigrade anhydrobiosis
Comparison of the effect of the presence of BHAM and MitoTEMPO during M. inceptum tun formation on return to full activity. For the comparison, ratios between fully active animals treated with BHAM (0.1 or 0.2 mM) or MitoTEMPO (0.01 mM) and proper control animals were calculated and analysed by application of the developed Linear Mixed Models (see electronic supplementary material, Table S4). The analysis was performed for different duration of the tun stage. The time windows of observations are indicated in minutes following the onset of rehydration. Full activity was defined as coordinated movements of animal body and legs (crawling). control 1, 0.3% methanol in the culture medium; control 2, the culture medium. *** p < 0.001 (see also Tab. S4). Arrows indicate statistically significant differences between the experimental groups, shown also in Fig. 2