Fig. 6

S-phase cell distribution and live observations and in regenerates, cutting level L2 + L3, midbody fragments. a–h′. Edu (pulse-chase) BrdU (pulse) stainings. Fluorescence microscopy images of S-phase cell distribution in regenerates. a–h. Anterior regeneration. a’–h′. Posterior regeneration; arrowhead in d′ points to EdU chased cells in the stock gut. i–r’. Light microscopy images of living specimens. i–r. Anterior regeneration. i′–r’. Posterior regeneration. White dashed lines circumscribe the shape of the animals. Black dashed lines indicate amputation site. Abbreviation: ve part of ventricle protruding outward. Scale bars: 100 μm (e, i–m, c′, e’–g’), 200 μm (a–d, f–h, n–r, a’, b′, d′, h′–r’)