Fig. 3

S-phase cell distribution and live observations and in regenerates, cutting level after pharynx (L1). a–h′. EdU (pulse-chase) BrdU (pulse) stainings. a–h. Anterior regeneration. a’–h′. Posterior regeneration. i–r’. Light microscopy images of living specimens. i–r. Anterior regeneration. i′–r’. Posterior regeneration. White dashed lines circumscribe the shape of the animals. Black dashed lines indicate amputation site. Abbreviation: pr proventricle. Scale bars: 100 μm (d, d′, g’), 200 μm (a–c, e–h, a’–b′, e’–h′, i–r’)