Fig. 8.

Real-time RT-PCR expression analysis of Sg-nag2 and Sg-cht5–1 on cDNA from parts of S. gregaria embryos. (a) cDNA was prepared from mRNAs isolated from parts of embryos at the age of 6, 8, 10 and 12 days: H, head; T, thorax; A+, abdomen with pleuropodia; A-, abdomen without pleuropodia. For each age the same number of body parts was used (5–10) and RNA was resuspended in the same volume of water. The size of the pleuropodium is indicated by the yellow dot. (b) and (c): expression of Sg-nag2 and Sg-cht5–1, respectively. Analysis of 3–4 technical replicates is shown. Expression in A + 8 (abdomen with pleuropodia at stage when the organs first become differentiated) was set as 1. Numbers above A+ expression is fold change from A- of the same age