Fig. 10

Real-time RT-PCR expression analysis of genes for lysozymes on cDNA from parts of S. gregaria embryos. cDNA was prepared from mRNAs isolated from parts of embryos at the age of 6, 8, 10 and 12 days. Labeling of the body parts is explained on the left of this Figure; see also an immage summary in Fig. 8a. For each age the same number of body parts was used (5–10) and RNA was resuspended in the same volume of water. Analysis of 3–4 technical replicates is shown. Expression in A+ 8 (abdomen with pleuropodia at stage when the organs first become differentiated) was set as 1. Numbers above A+ expression is fold change from A- of the same age