Fig. 5

The deutocerebrum and central olfactory system. a Frontal vibratome section of the posterior part of the brain showing the projection neuron tract, labeled against acetylated tubulin. b Magnification of the chiasm of the projection neuron tract with neurites projecting contra- and ipsilaterally (red arrows), labeled against acetylated tubulin (black-white inverted). c Magnification of the deutocerebral chemosensory lobe of the right brain hemisphere showing the peripheral arrangement of spherical to wedge-shaped glomeruli, labeled against acetylated tubulin (black-white inverted). d Horizontal view on the deutocerebral chemosensory lobe of the right brain hemisphere showing three RFamide-like immunoreactive neurons located in cluster 9/11 (asterisks), triple-labeled against acetylated tubulin (yellow-red), RFamide (green) and nuclei (cyan, D1) as well as against RFamide, separately (black-white inverted, D2). Insets (bottom left corner) show position and orientation of depicted sections in the brain. Abbreviations: dc deutocerebrum, dcl deutocerebral chemosensory lobe, hn/tm hemiellipsoid body/ terminal medulla complex, la lamina, lan lateral antenna 1 neuropil, lo lobula, maf a myoarterial formation a, og olfactory glomeruli, pb protocerebral bridge, pmpn posterior medial protocerebral neuropil, pnt projection neuron tract. Scale bars: (a) 50 μm, (b-d) 25 μm