Fig. 5

L. longissimus pilidium larvae and the influence of excitatory peptide on their ciliary beating. a SEM picture of a L. longissimus larva. b Experimental setup to record the ciliary beating and the influence of excitatory peptide on a microscope slide. c Light microscopic picture of L. longissimus larvae with a developing juvenile on a holding pipette. d Beating ciliary band of the apical lobe. e The same ciliary band as in D, after overexposure to 25 μmol/l EP2. f Boxplot of the ciliary beat frequency of the apical lobes and lateral lappets under normal conditions, after exposure to either 10 μmol/l EP1 or 5 μmol/l EP2, and after peptide washout. Bold horizontal line indicates median, box indicates upper and lower quartile, whiskers indicate variability outside the upper and lower quartile, circles indicate outliers. Lines within the graph indicate measurement points of individual larvae. P values were calculated with a paired t-test. Asterisks indicate significance with p < 0.02. al = apical lobe, at = apical tuft, bps beats per second, cb = ciliary bands, cs = cover slip, Ctrl = control, EP = excitatory peptide, gs = microscopic glas slide, hp. = holding pipette, dj = developing juvenile, l = larva, ll = lateral lappet, st = stomach