Fig. 7
From: Phylogenetic analysis and embryonic expression of panarthropod Dmrt genes
Expression of Doublesex (Dsx) in Tribolium (A, universal probe; B, female-specific probe. Note that the patterns appears strongly in half of the embryos, while it appears after prolonged staining time in the other half of the embryos. Figure 7B represents such latter case embryo.) and Parasteatoda (C-F, Dsx2 isoformsA,C,D; G-J, Dsx2 isoformB). Developmental stages are indicated. Panels A´-J´ represent DAPI stained embryos as seen in panels A-J. In all panels, anterior is to the left and ventral views, except panels C and E (lateral views) and F (dorsal view). Arrows in panel F point to single cells expressing the gene. Asterisks in panels H-J mark splitting expression in the fourth opisthosomal segment. Dashed lines in panel E/E´ mark the dorsal field. Abbreviations as in Fig. 4; A10, tenth abdominal segment; br, brain; O(x), opisthosomal segments. Note that the primordia of spider spinnerets are located on opisthosomal segments four (O4) and five (O5) (panels G-J)