Fig. 2

Conserved domains of Dmrt genes. The conserved domains are highlighted: the DM domain, the DMA domain, the oligomerization domain (OD) of pancrustacean Dsx genes, the Short-Conserved-Motif (SMC), and the peculiar reticulocyte binding domain-like motif (RBD). Asterisks (*) mark unknown sequence information. Question marks (?) indicate missing information about presence or absence of a domain. Whenever the core motif (SAF) of the SCM is derived, this is indicated with the different sequence in purple letters next to the SCM-domain box. Position of primers of cloned and sequenced gene fragments is indicated by red arrows. The region marked by the red arrows was used to synthesize anti-sense mRNA probes for in-situ hybridization experiments. Blue arrows mark the region of Pt-Dmrt_like that we cloned to verify the presence of the RBD-like motif. Red filled circles in front of gene names indicate genes that have been cloned in this study. The black filled circle indicates that we attempted to amplify this gene by means of RT-PCR (position of primers marked by black arrows), but did not succeed. Locations of primers for the different splice variants of Tribolium Dsx, Parasteatoda Dsx2 and Glomeris Dmrt11E are indicated in Fig. 3. Species abbreviations: Dm, Drosophila melanogaster; Dma, Daphnia magna; Ek, Euperipatoides kanangrensis; Gm, Glomeris marginata; Pc, Priapulus caudatus; Pt, Parasteatoda tepidariorum; Tc, Tribolium castaneum