Differential expression of myosin heavy chain isoforms in cardiac segments of gnathostome vertebrates and its evolutionary implications

Table 2 Results from peptide sequence quantification by ESI-Quadrupole-Orbitrap of the inflow and outflow segments of S. canicula.

Protein name	Abundance average (Inflow)	Abundance average (Outflow)	% Isoform (Inflow)	% Isoform (Outflow)
Slow myosin heavy chain 2	169549940.70	93548252.81	34.3%	8.3%
Myosin-6	211090171.21	229440263.67	42.7%	20.4%
Myosin, heavy chain 7, cardiac muscle	108968584.27	511231134.91	22.0%	45.3%
Myosin-7B-like	195823.66	271036094.03	0.0%	24.0%
Myosin-10-like	2071101.07	9660654.80	0.4%	0.9%
Myosin-9-like	877770.49	2696819.69	0.2%	0.2%
Myosin-11	1731453.90	9714374.82	0.4%	0.9%

Results from Myosin heavy chain isoform average abundance (arbitrary units) and its percentage by ESI-Quadrupole-Orbitrap of the inflow and outflow segments of S. canicula (n=3) compared with a C. milii database. Both samples contain four sarcomeric isomyosins. The fast-twitch MYH2 and MYH6 are more abundant in the inflow segments whereas the slow-twitch MYH7 and MYH7B are predominant in the outflow segments. A small proportion (1-2%) of the non-sarcomeric isoforms MYH9, MYH10, MYH11 was found in both segments

ISSN: 1742-9994