Fig. 3
From: Comparative and functional analysis of the digital mucus glands and secretions of tree frogs
Brightfield micrographs of cryo-histochemically stained mucus glands in the digital pads of Hyla cinerea. Per staining, an overview of a whole representative transverse section through the gland space is shown. Insets: magnified views of a dorsal (top) and a ventral (bottom) mucus gland; black arrowheads: positive staining, white arrowheads: equivocal staining, Scale bar = 25 µm; CRO Crossmons’s light green trichrome including Mayer’s haematoxylin and Alcian blue, OIO Oil Red O, PAS Periodic acid-Schiff, PAS-D Periodic acid-Schiff-Diastase, AB Alcian blue (pH =1, pH =2.5), NIN Ninhydrin-Schiff, COO Coomassie blue, BRO Mercuric bromophenol blue (pH =3.8, pH =4.6). CH Chromatophore, CO Collagen, DE Dermis, DP Digital phalanx, DU Mucus gland duct, ED Epidermis, MG mucus glands. OIO- and NIN-stained sections were imaged using differential interference contrast