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Fig. 2 | Frontiers in Zoology

Fig. 2

From: Also looking like Limulus? – retinula axons and visual neuropils of Amblypygi (whip spiders)

Fig. 2

Cobalt fills via lateral eyes in Amblypygi. Damon medius; frontal sections (a-d); sagittal view (e-h). a, b, Cobalt fills via two lateral eyes, accordingly two subunits (subunit a, b) of first (a) and second (b) visual neuropils are stained. Note dense arrangement with varicosities of Cobalt-filled profiles. In the tract between the first and second neuropil (arrow) it seems, that the fibres cross. Bars 50 μm. c, d, Cobalt fills via one lateral eye (different eye than in a, b), accordingly different subunit (subunit c) of the first (c) and second (d) visual neuropil are stained than in a, b. Bars 50 μm. e-h, Comparison of whole mount, μCT, and sections of same specimen. Two lateral eyes are filled with cobalt, hence two subunits of the first lateral eye visual neuropil are stained. Dorsally the first and second visual neuropil is stained with cobalt, but ventrally cobalt stopped in the first visual neuropil, and the second neuropil remained unstained. In whole mount (e; bar 250 μm) only the first visual neuropil is definitely visible. In μCT reconstruction (f; bar 250 μm) the first and second neuropil is visible. Most details become apparent in sections (g, h; bars 100 μm). L1, first lateral eye neuropil; L2, second lateral eye neuropil; a-c subunits of L1 and L2; arrow, tract connecting L1 and L2

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