Fig. 4
From: Neuromuscular transmitter candidates of a centipede (Lithobius forficatus, Chilopoda)
Colocalisation of synapsin- with glutamate-IR at body wall neuromuscular junctions. a-c: Confocal images (maximum projection of 18 images at 1 μm intervals) of dorsal body wall muscles 6a (intersegmental muscles of a segment with long tergite) labeled with antibodies against glutamate (a) and synapsin (b) displaying numerous co-labeled boutons (c) Arrows point to two motor nerves innervating the muscle. d-f: Confocal images (maximum projection of 28 images at 1 μm intervals) of ventral body wall muscles 2 h and 2 g labeled with antibodies against glutamate (d) and synapsin (e) displaying numerous co-labeled boutons (f, arrowheads).). g-j: Confocal images (maximum projection of 11 images at 1.6 μm intervals) of a ventral body wall muscle labeled with antibodies against glutamate (g) and synapsin (h). Presynaptic sites appear as aggregates of several small dots, most of which are co-labeled by both antibodies (j, arrowheads). Anterior is to the left in all panels. Scale bar: a-c 100 μm, d-f 50 μm, g-j 20 μm