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Fig. 3 | Frontiers in Zoology

Fig. 3

From: Neuromuscular transmitter candidates of a centipede (Lithobius forficatus, Chilopoda)

Fig. 3

Localisation of acelylcholine esterase staining (brown) and synapsin-IR(red). a-c Dorsal body wall muscle 6a and peripheral nerve stained for acetylcholine esterase (a) and immunolabeled for synapsin (b), c is a merge of a and b. Peripheral nerve is outlined in white, selected synaptic boutons from B are marked with arrowheads in all panels. d-f: Tibia of a walking leg stained for acetylcholine esterase (d) and immunolabeled for synapsin (b), f is a merge of d and e. Peripheral nerve 4 is outlined in white, selected synaptic boutons from e are marked with arrowheads in all panels. Arrow points to a trachea accompanying the leg nerve. Note absence of colocalization of AChE and synapsin. Muscle numbers are indicated. Anterior is to the left in a-c, distal is to the left in d-f. Scale bar 100 μm

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