Fig. 3

Functional chloroplast efficiency and abundance. a The chloroplast efficiencies (PAM values) of E. timida in each temperature treatment and E. cornigera. E. cornigera drops in photosynthetic efficiency more rapidly than any of the E. timida treatments. Each point is the average of three independent measurements following dark acclimation. E. timida at 25 °C is denoted by light green circles, at 21 °C by medium green circles and at 18 °C by dark green circles. E. cornigera at 25 °C is represented by blue squares. Lines connecting each point display the average rate of decrease. b Percent chlorophyll coverage throughout starvation for each examined species. This was computed by measuring the area (in each image, for each stack) that was covered in Digestive Gland Tubule (DGT) and the remaining area – designated as Non-Digestive Gland Tissue. The area covered by functional chloroplasts inside the DGT was divided by the total DGT producing a relative value, a percent of the DGT that is filled with chloroplasts. This value was averaged for each measurement taken (8 images/stack, 5 stacks/specimen, 3 specimens/time point totaling 120 measurements for species at each time point) and graphed here to show the overall trend for each species at each time point. E. timida 18 °C is shown by dark green circles, 21 °C by medium green circles and 25 °C by light green circles. E. cornigera is depicted by blue squares. Error bars are not depicted here for graph clarity and can be found in the text