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Table 1 Primary and secondary antibodies used in the study

From: Nervous system development in the Pacific oyster, Crassostrea gigas (Mollusca: Bivalvia)

Antibody

Host species

Source, Cat. #

Dilution

Acetylated α-tubulin

Mouse monoclonal

Abcam, 6-11B-1

1:2000

FMRFamide

Rabbit polyclonal

Immunostar, 20091

1:2000

5-HT

Rabbit polyclonal

Immunostar, 20080

1:2000

ChAT (choline acetyltransferase)

Goat polyclonal

Millipore, AB144P

1:500

VAChT (vesicular acetylcholine transporter)

Goat polyclonal

Millipore, ABN100

1:500

AlexaFluor 555 anti-goat

Donkey

Life Technologies, A21432

1:1000

AlexaFluor 488 anti-rabbit

Donkey

Life Technologies, A21206

1:1000

AlexaFluor 555 anti-rabbit

Donkey

Life Technologies, A31572

1:1000

AlexaFluor 647 anti-mouse

Donkey

Life Technologies, A31571

1:1000

AlexaFluor 555 anti-mouse

Donkey

Life Technologies, A31570

1:1000