Table 1 Antibodies used in this study
From: The complex simplicity of the brittle star nervous system
Antibodies used | Source | Host species | Dilution |
|---|---|---|---|
First antibodies | |||
Acetylated tubulin | Sigma (T6793) | Mouse | 1:1,000 |
Brn1/2/4 | [22] | Rat | 1:1,000 |
ELAV | [22] | Rabbit | 1:3,000 |
ERG1 | [18] | Mouse | 1:1 |
GFSKLYFamide | [20] | Rabbit | 1:1,000-1:5,000 |
Second antibodies | |||
Goat anti-mouse Cy3 | Jackson Immunoresearch (115-165-146) | Goat | 1:2,000 |
Goat anti-rabbit Cy3 | Jackson Immunoresearch (115-165-144) | Goat | 1:1,000 |
Goat anti-rabbit FITC | ThermoFisher Scientific (65-6111) | Goat | 1:200 |
Goat anti-rat FITC | GenWay (GWB-7B4D70) | Goat | 1:100 |
Synaptotagmin B | [21] | Rat | 1:5,000 |