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Fig. 3 | Frontiers in Zoology

Fig. 3

From: Developmental biology and potential use of Alboglossiphonia lata (Annelida: Hirudinea) as an “Evo-Devo” model organism

Fig. 3

Fluorescent lineage tracer injection and Anti-acetylated tubulin stained A. lata embryo. To show the broad lineage of DM cell, we injected the RDA in DM cell at stage 4b. DM cells divided into left and right mesodermal precursor cells. DM cells were divided to Ml and Mr at stage4c. After then, M lineage precursor cells organize the mesodermal lineage tissue and have overall function of mesoderm lineage. a To confirm the exact function of M lineage precursor cell in A. lata embryos, we injected left M cells at stage 6a. M cells have a function to develop mesodermal lineage tissue (muscle fiber, mesodermal prickle cell). N cells have a potential to develop neuronal tissue, presumptive neuronal ganglion. OPQ cells have function of neuro-ectodermal lineage, exterior region of N lineage. To confirm the separative lineage of N and OPQ, we injected double lineage tracer at stage 6a. N and OPQ cells have individual lineage in developing A. lata embryos; lg: left germinal band; rg: right germinal band; rmt: right M teloblast; lmt: left M teloblast; lnt: left N teloblast; lot: left O teloblast; lpt: left P teloblast; lqt: left Q teloblast. Scale bar in all images 100 μm. b To show A. lata as the experimental model, we stained late stage embryos using Anti-acetylated tubulin. At stage 10, Anti-acetylated tubulin stained nerve fiber in the everted proboscis. Out focused embryos show that Anti-acetylated tubulin has a role in ventral ganglion and peripheral nerve fiber. At stage 11, Anti-acetylated tubulin is expressed in overall part of embryo. We pseudo-colorized the DAPI stained ventral ganglion at stage 11 for optimizing the peripheral nerve and central nerve-ganglion. Scale bar in all images 100 μm

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