Fig. 6
From: The flipflop orphan genes are required for limb bud eversion in the Tribolium embryo
Larval Tc-RhoGEF2 RNAi phenotype. a Magnified view of the KT221 mutant phenotype displaying an inverted leg (arrow) and head appendage (arrowhead). b, c RhoGEF2 RNAi cuticles display more severe defects: in addition to inverted appendages of the head (arrowheads) and thorax (arrows) RhoGEF2 knockdown also results in segmentation defects, a malformed posterior abdomen (b dotted outline) and dorsal closure defects (c, outlined with dots). Extended focus in all pictures combines several optical sections. a lateral view; b ventral view; c dorsal view); b, c scale bar 100 μm