Fig. 3
From: The flipflop orphan genes are required for limb bud eversion in the Tribolium embryo
The “flipflop syndrome”. a-i Larval RNAi phenotypes of Tc-ff1 a-c, Tc-ff2 d-f and Tc-ff1/ff2 g-i parental double-knockdown. a-i The enigmatic phenotype of inverted head appendages (arrowhead) and legs (arrows) in different combinations. In addition, inverted abdominal segments (e, circle) and dorsal openings (c, h, dotted line) are detected. f, i In stronger cuticle phenotypes cuticle remnants also show inverted appendages. A1 abdominal segment 1; T1 thoracic segment 1; scale bar 100 μm; all panels in all pictures: anterior to the left