Fig. 1
From: The flipflop orphan genes are required for limb bud eversion in the Tribolium embryo
Larval Tc-flipflop RNAi phenotype. (a) Wildtype cuticle. All appendages develop as everted structures. Each thoracic segment bears one pair of visible legs. (b-b′) Tc-ff RNAi phenotype. (b) Surface view, (b´) optical section. (b) In the thoracic segments 2 and 3, one leg is everted normally. Of the other legs, only the coxa is visible as an outer structure (circle) while the remaining leg is internalised (b′, arrows). (c, d) Gene organisation of Tc-ff1 (c) and Tc-ff2 (d) genes including NOF (non-overlapping-fragment) positions used for RNAi experiments. A1 abdominal segment 1; Ant antenna; Md mandible; T1–3 thoracic segment 1–3; ug urogomphi; scale bar (a-b′) 100 μm; all panels in all pictures: anterior to the left