Fig. 5
From: Morphogenesis of honeybee hypopharyngeal gland during pupal development
Gland units in pupal hypopharyngeals glands are composed of three cell types. Cryo-sections of hypopharyngeal glands were labeled with fluorophore-tagged phalloidin (green) and DAPI (blue) and imaged by confocal serial sectioning. Gamma correction was set to 0.5 for phalloidin images. Gland units were examined at developmental stages P2/P3 (a-c), P4 (e-g), and P5 (i-l). d, h and m Schematic presentations of the spatial arrangement of the secretory cell (SC), duct cell (DC), and accessory cell (AC) at developmental stages P2/P3, P4, and P5, respectively. a,b An optical plane visualizing a P2/P3 gland unit in longitudinal section, with the three cell types highlighted in (b). c The area outlined in a at higher magnification, showing the SC process (arrowheads) contacting (arrow) the distal end of the future ductule. e and f An optical plane visualizing a P4 gland unit in longitudinal section, with the three cell types indicated in (f). g The area outlined in e at higher magnification, showing the F-actin-rich terminus (arrow) of the short secretory cell process (arrowheads) that extends toward the future ductule. i-k Three image planes with an inter-plane distance of ~1.2 μm through an acinus at developmental stage P5. The indicated area is shown at higher magnification in the insets below. i’-k’ Lower regions of images i-k, with secretory cell (SC), accessory cell (AC), and duct cell (DC) outlined. Nuclei of the three cell types are indicated by asterisks. Insets in i’-k’ The distal part of the duct cell (arrowheads) is enclosed by the accessory cell. Arrowheads indicate the plasma membrane of the duct cell. l Maximum intensity projection of the entire image stack, representing a thickness of ~5 μm and showing three gland units. The presence of an acessory cell (ACn) is consistent for all secretory cell / duct cell assemblies. Bars, 20 μm