Fig. 2

Methods. a Toluidine-blue stained cross-section of 42 days starvation specimen of E. timida from Blanes population, the rectangle indicates the area seen in B; (b) magnified area showing digestive tubule epithelium with chloroplasts (tubules indicated with arrowheads) in the parapodia at higher magnification; (c) Lugol’s solution-stained section of a 0 days starved E. timida from the Elba population, the digestive gland tubules have been isolated by deleting the background, non-digestive tissue; (d) Conversion to greyscale image in Adobe Photoshop; (e) Grey shades reduced to 5 for final starch counting, the dark black coloration shows the Lugol’s stained starch. Scale bars: A = 50 μm; B = 25 μm; C-E = 20 μm