Fig. 3

DiO (a, b, d, and e) and DiI (c) labelling via both eyes of Opilio canestrinii. (a-c, fluorescence microscope; d, e, CLSM; dorsal is up). a DiO labelled eye, eye nerve bundles, eye nerve, and visual neuropils; no distinction between first and second neuropil possible. Bar 200 μm. b DiO labelled eye nerve bundles, eye nerve, and visual neuropils; no distinction between first and second neuropil possible. Bar 200 μm. c DiI labelled eye nerve bundles, eye nerve, and visual neuropils; no distinction between first and second neuropil possible. Bar 200 μm. d DiO labelled eye nerve, and visual neuropils; no distinction between first and second neuropil possible. DAPI labelled cell bodies in green. Same specimen as in A. Bar 100 μm. e DiO labelled eye nerve, first, and second visual neuropil. Neuropil border between first and second neuropil visible (arrowheads), in second visual neuropil fewer DiO. Same specimen as in B. Bar 100 μm. EN, eye nerve; ENB, eye nerve bundles; EYE, eye; M, median eye visual neuropil