Fig. 5
From: Formation of the germ-disc in spider embryos by a condensation-like mechanism
The effect of alpha-amanitin injections on the mid-blastula transition. a Stills from Additional file 10: Movie 10. P. tepidariorum embryos have been injected with water (control) or alpha amanitin (α-AM) at stage 1. Five hours after injection (5 h) both embryos have proceeded normal embryonic developed. The control embryo has formed a distinct germ-disc 16 h after the injection and has reached stage 4. The alpha-amanitin injected embryo has arrested at late stage 2 and has not formed a germ-disc. b Stage 1 embryos of four different cocoons from four different spider females have been injected with water (control #1 - #4) or alpha-amanitin (α-AM #1 - #4). While most of the control embryos develop normally and do form a germ-disc, most of the alpha-amanitin injected embryos do not form a germ disc and have arrested at late stage 2. c and d The injection of alpha-amanitin does not affect cellularization. e PCR for Pt-hedgehog (Pt-hh) and Pt-polyubiquitin (Pt-PolyUb) on water (control) and alpha-amanitin (α-AM) injected embryos. While the control gene (Pt-PolyUb) is expressed in both samples, the expression of Pt-hh is not up regulated in alpha-amanitin injected embryos. See supplementary material for more details