Fig. 3
From: Formation of the germ-disc in spider embryos by a condensation-like mechanism
The influence of the actin and microtubule cytoskeleton on the formation of the germ-disc and the integrity of the blastoderm. Stage 2 and 3 control embryos (a and c) and stage 2 and 3 cytochalasin D injected embryos (b and d) have been re-injected with FITC-WGA two hours after the first injection. The integrity of the blastoderm (b', b'') and of the germ-disc (d', d'') is disrupted in CD injected embryos. Arrowheads in d” indicate the rupture of the blastoderm. e, f 5 h after the initial injection and after the formation of the germ-disc (the initial injection was during the course of germ-disc formation at early stage 3), a control embryo (e) and a colchicine injected embryo (f) have been re-injected with FITC-WGA. Colchicine does not block germ-disc formation (Additional file 6: Movie 6) but blocks further cell divisions (compare the number of cells (indicated by the white dots and the numbers in e” and f”) within a 100 μm2 (indicated by the dashed boxes in e” and f”)). Boxed areas in a’ - f’ are magnified in a”- f”. The asterisk in c-f marks the primary thickening. Dotted line in f indicates the anterior rim of the germ-disc. The two embryos shown in a and b, c and d, e and f are from the same cocoon, respectively. Scale bar is 50 μm in all panels